OCT4 Antikörper (N-Term)

Produktnummer ABIN127203

Dieser Anti-OCT4 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von OCT4 in WB, IP und IHC (p). Geeignet für Human, Maus, Ratte, Rind (Kuh) und Schwein.

Kurzübersicht für OCT4 Antikörper (N-Term) (ABIN127203)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-OCT4 Antikörper

Bindungsspezifität: N-Term

Spezifität: The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the N-terminus of mouse Oct-4.

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL. Positive Control: Rat kidney tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 30 % Glycerol, 0.5 % BSA

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: OCT3/4

Hintergrund: Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1

Gen-ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance